Ultra High Performance Liquid Chromatograph
In response to the wide variety of customer demands for improving analytical workflow, Shimadzu is constantly introducing advancements in high-performance liquid chromatographs, with features such as superior reproducibility of retention times or for ultra-trace sample injection volumes, fast multianalyte analysis, low carryover, automatic sample pretreatment, high-sensitivity detection, and longer lasting consumables.
- Description
Description
Description
Using network technology based on the Internet of Things (IoT) and the cloud to automatically collect information about instruments within laboratories, it is now possible to not only monitor the operating status of instruments, but also ensure instruments can always be used in their optimal state. By merging and making further advancements to such state-of-the-art technologies, the Nexera series offers the unprecedented experience of analytical instruments thinking on their own to better support the analysis workflow of customers.
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Automated support functions utilizing digital technology, such as M2M, IoT, and Artificial Intelligence (AI), that enable higher productivity and maximum reliability. |
A New Benchmark of Intelligence
Nexera systems are equipped with startup, self-diagnostics/self-recovery, mobile phase monitoring, and other critical functionality for ensuring the system is always in optimal condition during analysis. That maximizes the ratio of operating hours during which the system is operated in good condition and automates the tedious processes which were previously performed by operators. Consequently, it contributes significantly to improving operating efficiency.
Fully Automated Process Flow Achieves Long-Term Data Reliability Long-term reliable data acquisition is achieved by incorporating the operating knowhow of expert analysts for the entire process flow, from instrument startup until analyses are finished. Systems also include many other functions for automatically eliminating factors that can cause problems.
Mobile Phase Level Monitor Prevents Running Out of Mobile Phase during Continuous Analysis
This monitor uses weight sensors (optional) to monitor the level of mobile phases, autosampler rinse solutions, or other solutions (up to 12 solutions) in real time. If the remaining solution level is less than required when starting an analysis, then a message is displayed to notify the operator. The operator is also notified if there is a risk of running out of the solution during an analysis. A smart device can be used to check the current level of each bottle, which makes it easier to decide in advance when to replenish bottles.
A New Benchmark of Efficiency
Functionality is included for maximizing throughput and increasing analytical process efficiency to the next level, such as functionality for ultra fast injection and low carryover. Also, IoT technology is used to ensure reliable instrument operation and contribute to laboratory asset management.
LC-MS Analysis Process Efficiency Improved by Ultra Fast Injection and Non-Stop Analysis of up to 17,000 Samples
The SIL-40 series offers about two times faster injection speeds than previous models. By using plate changers, up to about 17,000 samples can be analyzed continuously.* Plate changers provide powerful support for LC-MS high-throughput screening and analysis.
* When using three plate changers with 384-well plates
A New Benchmark of Design
Offers both exceptional LC performance and also space and power savings. Due to the broad product line, optimal solutions for satisfying customer objectives can be offered, while also maximizing utility from laboratory equipment.
| System Controller |
| SCL-40 and CBM-40/40lite |
The SCL-40 system controller includes a touch panel that can be used to directly control the instrument without using a computer. That makes it easy to prepare for analysis even without a control computer. An optional mobile phase controller can be installed in the reservoir tray area. It helps prevent running out of mobile phase by measuring the weight of mobile phase bottles in real time and sending the results to a smart device or computer. SCL-40 and CBM-40 Specifications
| Number of units controllable | 8 (or 12 using option) |
| Event inputs/outputs | 1 input and 2 outputs |
| Solvent Delivery Unit |
| LC-40D/40B Series |
In addition to four parallel-type double plunger models for different allowable upper pressure limits, XR and X3 models feature smaller gradient delay volumes and a dual pump that enables both ultra highspeed and high-pressure gradients using the same system. A wide variety of other solvent delivery environments are available as well, such as for low-pressure gradients or mobile phase blending.
LC-40D XR and LC-40B XR Specifications
| Pump type |
Parallel-type double plunger |
| Flow rate range | 0.0001 to 3.0000 mL/min (1.0 to 70 MPa) 3.0001 to 5.0000 mL/min (1.0 to 44 MPa) 5.0001 to 10.0000 mL/min (1.0 to 22 MPa) |
| Autosampler |
| SIL-40/40C Series |
These models achieve ultra low carryover of 0.0003 % or less. Ultra fast injection, automatic pretreatment functions, and other features help improve analytical efficiency even further. The optional dual-injection function enables simultaneous data acquisition using two completely different analysis systems, by installing two internal injection ports and providing completely independent flow channels downstream from those ports.
SIL-40 XR, 40C XR, 40C XS, and 40C X3 Specifications
| Injection method |
Total volume injection (standard) Loop injection (optional) |
| Injection volume settings range | 0.1 to 50 µL (standard) 1 to 2,000 µL (optional) |
| Plate Changer |
The 170 mm width results in a significantly smaller installation footprint. It holds up to 14 microplates or seven 1.5-mL vial plates. Up to three plate changer units can be linked to hold, including in the autosampler, up to 44 microplates or 16,896 samples (using 384-well plates).
| Number of samples processed |
Including 2 autosampler plates: 108 to 6,144 Including 2 autosampler plates and 3 linked plate changers: 276 to 16,896 |
| Column Oven |
| CTO-40C/40S |
The 130 mm wide narrow model with forced air circulation has a maximum temperature of 85 °C and the 260 mm wide standard model has a maximum temperature of 100 °C. Both can hold 300 mm long columns and include internal connectors that recognize gradient mixers. (The narrow model includes one connector and the standard model two.) Ovens can also be installed with optional preheating tubes.
| Temperature control method |
Forced air circulation |
| Temperature control range | CTO-40C: 10 below room temperature to 100 °C CTO-40S: 10 below room temperature to 85 ° |
| UV-VIS Absorbance Detector and PDA Detector |
| SPD-40/40V and SPD-M40 |
These newly developed models offer improved baseline stability for room temperature fluctuations and improved linearity. The photodiode array (PDA) detector includes a UV cutoff filter that improves quantitative accuracy of photolytic compounds. Cells and lamps have ID codes that help improve traceability.
| Light source |
SPD-40: D2 lamp SPD-40V, SPD-M40: D2 lamp, W lamp |
| Wavelength range | SPD-40: 190 to 700 nm SPD-40V: 190 to 1,000 nm SPD-M40: 190 to 800 nm |
| Ultra High Performance Liquid Chromatograph Nexera |
| Dual Injection System |
The dual injection system allows data to be collected at the same time under two different analysis conditions on a single platform, making analysis operations more efficient and space-saving. Two chromatograms from one sample under different analytical conditions are stored in one data file. It not only facilitates data management, but also ensures traceability of samples and data. There is only one method file and batch file, and no cumbersome analysis setup operations are required. It is suitable for the study of food functional ingredients, the quantification of multiple ingredients in the field of metabolomics, and the analysis of pharmaceutical impurities, which is difficult to analyze simultaneously under a single condition.
| Major component units |
CBM-40, LC-40B XR×2, DGU-405, SIL-40C XR, CTO-40C, SPD-M40, SPD-40V, LabSolutions LC, and others |
| Ultra High Performance Liquid Chromatograph Nexera |
| Method Scouting System |
Method-scouting systems combine the speed of analysis method and schedule generation with dedicated software with the reliability of robust column switching systems to provide strong support for method exploration. The method search including SFC analysis and simple method examination using integrated LC are also supported. In conjunction with LabSolutions DB/CS’ s multi-data reporting function, the system automatically outputs the optimal analysis conditions based on customer criteria in order of score. In addition, each chromatogram can be displayed in a list, and it is easy to confirm the optimum analytical conditions visually, and the time and effort required from the examination of analytical conditions to the confirmation of results can be greatly reduced.
| Major component units |
SCL-40, LC-40DX3×2, DGU-405×2, SIL-40C X3, CTO-40C, SPD-M40, LabSolutions LC, and others |
| Semi-Preparative Supercritical Fluid Chromatography System |
| Nexera UC Prep |
Nexera UC Prep is a preparative supercritical fluid chromatography system created by the combination of Nexera UC’s superior fundamental technology and innovative new technology. By using our unique gas-liquid separation technology “LotusStream Separator”, we can achieve a high recovery rate even for volatile components by suppressing a decrease in recovery rate due to the scattering of eluate during CO2 vaporization. The dedicated software, which enables intuitive parameter settings, helps ensure preparation for the targeted, regardless of skill. This space-saving benchtop model includes a carbon dioxide pump that does not require an external chiller. While the system is compact and can be installed in any environment, it achieves both high recovery rate preparative processing and excellent operability, and promotes the efficiency of preparative processing.
| Major component units |
LC-40P SF, CO2 Cooling Unit, SFC-40P, FRS-40, HEX-40, CBM-40, LC-20AP, LC-20AR, CTO-40C, SPD-M40, DGU-403, FCV-20AH2, LabSolutions LC, Prep Solution, and others |
| Preparative Purification Liquid Chromatograph |
| Nexera Prep |
These preparative purification LC systems offer expandability for collecting up to 3240 fractions in 10-mm diameter test tubes by linking up to six new LH-40 liquid handler or new FRC-40 fraction collector units, which require about 50 % less installation space than comparable competing products, and a column hub that can hold up to six columns and four flow channel selection valves. LH-40 liquid handlers can be used not only as a fraction collector, but also as an autosampler equipped with a liquid level detection function (optional) or for seamlessly checking purity by reinjecting fractions after fractionation (optional). That supports achieving even higher efficiencies for preparative purification operations.
| Major component units |
CBM-40, LC-20AP×2, DGU-405×2, SPD-M40, LH-40, Column Hub, LabSolutions LC, and others |
| Anion Suppressor Ion Chromatograph |
| HIC-ESP |
The HIC-ESP is a new anion suppressor ion chromatograph with built-in electrodialytic suppressor, boasting the same low carryover and excellent injection precision characteristic of Shimadzu HPLCs to bring you highly-reliable results. The newly developed anion suppressor prevents peak spreading and achieves high sensitivity, providing stable functionality even over long periods of use. The HICESP is suitable for applications in a wide range of fields including environmental science, medicine, chemistry and food science.
| Major component units |
CBM-40lite, LC-20ADSP, DGU-403, SIL-20A, CTO-40S, CDD-10AVP, LabSolutions LC, and others |
| Nexera Application System |
| Bromate Analysis System |
This bromate analysis system uses the ion chromatography—post-column absorbance detection method (tribromide ion method), based on the Japanese ministerial ordinance regarding water quality standards. It is capable of high-sensitivity detection at the µg/L level or less, using Shimadzu’s renowned post-column reaction technique and a high sensitivity UV-VIS detector. For the post-column unit, a piping parts kit specifically for the tribromide ion method is available, in which a reaction reagent mixing device (Japanese Patent No. 4082309) is adopted in order to efficiently mix high-concentration reaction solutions. The system enables simultaneous quantitation of halogen oxides. Iodic acid ions and chlorous acid ions are simultaneously quantitated as halogen oxides.
| Major component units |
CBM-40, LC-40D ×2, LC-20Ai, DGU-405, SIL-20AC, inert kit, CTO-40C, SPD-40, LabSolutions LC, and others |
| Nexera Application System |
| Anionic Surfactant Analysis System |
This anionic surfactant analysis system is based on the Japanese ministerial ordinance regarding water quality standards. The fluorescence detector (RF-20Axs) achieves an unprecedented sensitivity, with a water Raman S/N ratio of at least 2,000. With grouping and group calibration functions, the system can perform quantitative analysis by totaling the peak area values for multiple isomers with differing alkyl chains. If an MS detector is used in the system, more abundant qualitative information can be obtained on a variety of surfactants.
| Major component units |
SCL-40, LC-40D, DGU-403, SIL-40C, CTO-40C, RF-20Axs, LabSolutions LC, and others |
Note: For systems using an MS detector, contact your Shimadzu representative separately.
| Nexera Application System |
| Cyanic Analysis System |
This high-sensitivity analysis system for cyanide ions and cyanogen chloride uses the ion chromatography—post-column absorbance detection method (4-pyridine carboxylic acid-pyrazolone method), based on the Japanese ministerial ordinance regarding water quality standards. It enables high-sensitivity simultaneous analysis of cyan and cyanogen chloride by their chemical form. The system adopts a column oven with a cooling function to achieve thermal recycling, in which the reaction heat from the second stage is reused for the first stage reaction. It is highly sensitive, with a detection limit (S/N ratio = 3) of 0.0001 mg/L (CN value). It can easily measure not only the standard value (0.01 mg/L) but even 1/10th of that concentration. Thanks to Shimadzu’s proprietary high performance columns, analysis can be performed in 10 minutes or less per sample.
| Major component units |
CBM-40, LC-40D ×3, LC-20Ai, DGU-405, SIL-20AC, inert kit, CTO-40C, SPD-40, LabSolutions LC, and others |
| Nexera Application System |
| Organic Acid Analysis System |
Shimadzu’s unique post-column pH-buffered electroconductivity method is ideal for the selective and highly sensitive detection of organic acids. Compared to conventional methods, such as the UV short wavelength method or a simple conductivity method, this system improves quantitation reliability. Compared to the postcolumn VIS absorption detection methods using pH indicators, this system has higher sensitivity, better linearity, and is easier to use. Complex samples (which usually require troublesome pretreatment) can be analyzed after simple pretreatment techniques such as dilution and filtration.
| Major component units |
CBM-40, LC-40D×2, DGU-403, SIL-40C, CTO-40C, CDD-10AVP, LabSolutions LC, and others |
| Nexera Application System |
| Nexera GPC System |
By combining the superior solvent delivery and sample injection performance of the Prominence series with a temperature-controlled detector, this system achieves rapid baseline stabilization and outstanding reproducibility of analytical results, which results in providing highly reliable data. Convenient features, such as an overlapping injection function and automated analysis workflow, help increase productivity for routine GPC measurements. The system is also able to recycle mobile phase from intervals where no components are eluted,* which minimizes any environmental impact.
* Using a solvent recycle valve (optional).
| Major component units |
CBM-40, LC-40D, DGU-403, SIL-40C, CTO-40C, RID-20A, LabSolutions LC, LabSolutions GPC software, and others |
| Columns for Ultra High Performance Liquid Chromatograph |
| Shim-pack Velox ™ LC Columns |
Designed to maximize performance of LC systems, Shimadzu’s Shimpack Velox columns with core shell technology enable you to achieve increased separations and faster analysis times on any LC platform. Whether developing a high efficiency LC separation method, transferring an existing method for increased throughput while maintaining resolution, or are trying to improve the resolution of a complex separation, Shim-pack Velox columns will satisfy your needs.
| Stationary phase |
C18, SP-C18, Biphenyl, PFPP, HILIC |
| Particle size | 1.8µm, 2.7µm, 5.0µm |
| Column I.D. |
2.1mm, 3.0mm, 4.6mm |
| Columns for Ultra High Performance Liquid Chromatograph |
| Shim-pack Arata ™ LC Columns |
Even for LC columns that claim to be designed for basic compounds, adequate resolution often can not be obtained due to problems such as leading of highly polar basic compounds, peak shape deterioration of acidic compounds, or long equilibration time required for low ionic strength acidic mobile phase. All of these issues have been solved with Shim-pack Arata that was specifically designed to give unmatched peak shape for basic compounds.
| Stationary phase |
C18, Peptide C18 |
| Particle size | 2.2µm, 5.0µm |
| Column I.D. |
2.0mm, 3.0mm, 4.6mm |
